FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773948
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12750.039.031091
GTATCAA30350.038.787181
GTGGTAT5300.034.9679951
TATCAAC36050.032.8405572
TGGTATC5600.031.8648642
ATAGGAC652.4069363E-531.6763113
CAACGCA39700.029.994095
AACGCAG40500.029.4016136
TCAACGC41600.028.7891484
ATCAACG42550.027.9850863
ACGCAGA45300.026.2862117
CGCAGAG46050.025.7819128
AGAGTAC45850.025.51852811
CAGAGTA45950.025.46299410
GCAGAGT47300.025.0989519
CTCTTAT1001.627238E-524.0243111
TCTTATA1001.6288675E-524.0212062
GGTATAC750.002053811422.8802931
TAGATCG1004.492224E-420.5896035
CTTACTC1206.6516564E-520.0176723
Produced by FastQC (version 0.11.5)