FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773948
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8760.11318589879423424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6400.040.1731071
GTATCAA37850.039.4888731
TGGTATC6650.038.652852
GGTATCA15550.037.257221
TATCAAC44150.033.767622
CAACGCA46450.032.0997435
AACGCAG47200.031.5896846
TCAACGC48150.031.1047734
ATCAACG48600.030.6757283
ACGCAGA51450.029.048737
AGAGTAC52450.028.03740911
CAGAGTA52650.027.93090610
CGCAGAG53050.027.784928
GCAGAGT54300.027.2715599
GAGTACG61150.020.51685112
AGTACGG60450.020.47090313
GTCTTAT1056.28428E-419.5891721
TTATACC1056.2952284E-419.584114
GAGTACT11500.019.37499412
ATTACAC900.00595641419.0401063
Produced by FastQC (version 0.11.5)