FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110533
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGGA200.007253180751.93984637
CAACTAG200.00728701751.87874235
GTATATA200.007420896651.6404271
CACTAAC200.00742221851.6381032
CTTATAC1800.049.727821
TATACAC1750.049.1769263
TTATACA1950.045.9005362
GGTATCA12200.043.456971
GTATCAA34500.042.7093731
TATCAAC38700.038.33942
GTGGTAT4150.038.1599921
ATACACA2650.036.3723684
TACACAT2700.035.6988075
TCGTATG1800.034.69099440
ACTGATA400.002624002334.42542
AGGGGGG2450.034.40567470
AGAGTAC43150.032.78251311
CAGAGTA43300.032.5894610
TGGTATC4800.032.273812
GCAGAGT46200.031.7357549
Produced by FastQC (version 0.11.5)