FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069082
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACAAACTCCTCGGACTTTCGGCGGAGGGACCAGGGTGGAGATCAAA47220.4416873541973394No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46470.43467198961351894No Hit
AGCCAGGACAGTCTCCACAGCTCCTAATCTATTTGGGCTCTTATCGGGCC37660.3522648403022406No Hit
GTTTGTAGACCTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAGT37490.3506746909965746No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35120.32850613891170183No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32890.30764712154914214No Hit
GGCACAGACTTCACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTA27110.25358204515649874No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26230.24535068404481603No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26150.2446023784892085No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25920.24245100001683687No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25880.2420768472390331No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTC25350.2371193229331333No Hit
GTCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCAAGACTATGC25110.23487440626631073No Hit
GTCTTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTCCACAGCTCCTAATCTATTTGGGCT24570.22982334376595998No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23730.22196613543208096No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23320.21813106945959243No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT22870.21392185070930014No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGACTTCACACTGAA22410.20961909376455687No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTCTTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTC22130.20700002431993056No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG20800.1945594444579555No Hit
GTCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATAAGAGCCCAAATAGATTAGGAG20680.19343698612454424No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20140.18838592362419349No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19670.18398962848499928No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAGTCTGTGCCTGATCCACTGCCA19350.18099640626256921No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAGCCCAAATAGATTAGGAGCTGTGGAGACTGTCCTGGCTTCTGCAGGTA19020.17790964584568816No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18780.1756647291788656No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18500.17304565973423927No Hit
CCCCTGGAGAACCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG18400.17211027778972987No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18330.1714555104285733No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC18040.16874290278949602No Hit
CTCCTGCATAGTCTTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTCCACAGCTCCTAAT18010.1684622882061432No Hit
GTTCAGTGGCAGTGGATCAGGCACAGACTTCACACTGAAAATCAGCAGAG17460.16331768751134149No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17050.15948262153885295No Hit
ATCCAAGACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC17030.1592955451499511No Hit
GTAATATACGGCCGTGTCCGCGGCGGTCACAGAGTTCAGCCTCAGGGAGAACTGGTTCTCGGACATGTCTACCGA17000.15901493056659827No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGACTCAGTCTCCAGTCTCCCTGCCCGTCACCC16690.1561152465386191No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15470.1447035868156044No Hit
GTTTATTACTGCATGCAAGGTCTACAAACTCCTCGGACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA15310.14320697570438937No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGACTCAGTCTCCAGTCTCC15300.14311343750993843No Hit
GTCTCCACAGCTCCTAATCTATTTGGGCTCTTATCGGGCCTCCGGGGTCC15110.14133621181537057No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14960.13993313889860648No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGACTCAGTCTCCAGT14920.1395589861208027No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGA14800.13843652778739143No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14570.1362851493150198No Hit
CAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAGTCTGTGCCTGATCCACTGCCA14500.1356303819538632No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14460.13525622917605945No Hit
CTTATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATCAGGC14430.13497561459270663No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14300.13375961806484443No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14130.13216946875917843No Hit
GTCTCCAGTCTCCCTGCCCGTCACCCCTGGAGAACCGGCCTCCATCTCCT14130.13216946875917843No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14120.1320759305647275No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13680.12796025000888614No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13570.12693132986992578No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13520.1264636388976711No Hit
GTGGAGACTGTCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCA13500.1262765625087692No Hit
GACTTCACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTACAAACT13430.12562179514761262No Hit
GACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT13290.12431226042529946No Hit
TTATTACTGCATGCAAGGTCTACAAACTCCTCGGACTTTCGGCGGAGGGA13290.12431226042529946No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13240.12384456945304476No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG13150.1230027257029863No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12850.12019657986945809No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12840.12010304167500715No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12720.11898058334159588No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGTTCTCCAGGGGTGACGGGCAGGGAGACTGGAGA12470.11664212848032238No Hit
GAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTACAAACTCCTCGGACTTTCGGCGGAGGGA11980.11205875695222631No Hit
GTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTACAAAC11920.11149752778552066No Hit
TCTCCACCCTGGTCCCTCCGCCGAAAGTCCGAGGAGTTTGTAGACCTTGCATGCAGTAATAAACTCCAACATCCT11820.11056214584101126No Hit
AGTCAGAGCCTCCTGCATAGTCTTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTCCACAG11530.107849538201934No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11430.10691415625742459No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11430.10691415625742459No Hit
ACCCTGGTCCCTCCGCCGAAAGTCCGAGGAGTTTGTAGACCTTGCATGCAGTAATAAACTCCAACATCCTCAGC11180.1045757013961511No Hit
GTGTGAAGTCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG11120.10401447222944545No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11110.10392093403499451No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11030.10317262847938699No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA10940.10233078472932852No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10920.10214370834042664No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT10790.10092771181256444No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10720.10027294445140784No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGA601.448609E-553.17893670
GTCTAAC200.00748473251.5285381
ACCGATT556.038426E-446.41070670
TCGTATG1200.040.43717640
GTATCAA17600.038.4512181
CTCGTAT1100.037.79176339
GCTAATC1100.037.60548433
GGTATCA8500.035.9689371
GTGGTAT2950.034.93461
TATCAAC20400.033.1720472
TGGTATC3300.032.2688872
CGTATGC1400.032.21662541
TGTTAGA8400.031.14766370
ATCAACG22000.031.0718173
ACGATTA450.004518797630.80927540
TAATCTC1359.094947E-1230.6688635
TCAACGC22400.030.3636114
CAACGCA22450.030.1429755
AACGCAG22700.029.6596786
CGAGACT1650.029.15154623
Produced by FastQC (version 0.11.5)