FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456168
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7480.16397467599656268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAATC301.1516022E-768.812123
ACGATAG300.002595652167.3142870
TCATAAT353.3401193E-758.9947552
GATACTT502.8959507E-453.85142570
GTCATAA408.379375E-751.6373941
TAATCAA408.4111343E-751.6090935
TACCAAT200.007435931451.6090934
ATAATCA451.8941482E-645.874754
TAGATTT607.117659E-444.87618670
GTGGTAT2150.043.2313041
GGTATCA6300.042.0749131
GTATCAA15350.040.1437261
GAGTATG350.001363667839.3298387
GATTCTA350.001363667839.3298389
GATAGGG350.001363667839.3298382
AAACTAT557.512732E-637.5421146
TATCAAC17300.035.8060652
CAATTAA601.3609277E-534.4249271
GTGTATG501.8836191E-434.4249271
ACCTAAG400.002628210734.40983615
Produced by FastQC (version 0.11.5)