FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680372
Sequences flagged as poor quality0
Sequence length20-76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12950.19033705090744474No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGT150.002320299669.25970539
CTTATAC150.002394812668.709871
ATACAGT200.007479178751.53618217
GTAGGAT200.00748787851.5210344
ACTAGCT200.00748787851.5210343
GTATCAA23050.039.347951
TATCAAC26150.034.8070762
GGTATCA10000.034.6984831
GTGGTAT4100.034.354931
TATAAGA400.002652648134.3473552
TGTAAGT400.002652648134.3473554
AACGCAG28750.031.7834456
TGGTATC4550.031.7052522
AGAGTAC28050.031.35653911
CAGAGTA28450.030.91567410
CAACGCA30050.030.5205275
GCAGAGT30000.030.2345649
ACGCAGA30500.029.849377
TCAACGC30750.029.8235594
GTGCTAC703.960982E-529.4470841
Produced by FastQC (version 0.11.5)