FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1401863
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCACTCACAGAGGTCAGCTTCAGGGAAA41840.2984599779008362No Hit
GTGTATTACTGTGCGGGGTCTGTCGCCCGAGACTTGGAGTGGTTATTTGG39460.28148256998009075No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC32430.2313350163318384No Hit
GTCTCTGGTGGCTCCGTCACCAGTAATAGTTACTACTGGGGCTGGATCCG27210.19409885274095973No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26180.1867514871282001No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT26130.186394818894571No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC25040.1786194514014565No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23860.1702020810878096No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21200.15122733105874112No Hit
CAGTAATACACAGCCGTGTCTGCGGCACTCACAGAGGTCAGCTTCAGGGA19420.13852994194154492No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA19100.13624726524631867No Hit
GTATTACTGTGCGGGGTCTGTCGCCCGAGACTTGGAGTGGTTATTTGGGCCCCATAGCTGGTTCGACCCCTGGGG18740.1336792539641891No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18430.13146791091568863No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC18320.1306832408017046No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17700.12626055470470368No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG17250.12305054060204172No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG16490.11762918345087929No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16180.11541784040237885No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15990.11406250111458824No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15780.11256449453334598No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA15660.11170849077263613No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15340.10942581407740984No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15230.1086411439634258No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG14860.10600179903457042No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14850.1059304653878446No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTGCACACGTCCGAGAACCAGTTTTCCCTGAAGCTGA14740.10514579527386057No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14540.10371912233934413No Hit
CACCATATCCGTGCACACGTCCGAGAACCAGTTTTCCCTGAAGCTGACCT14470.1032197868122634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)