FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1401863
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCACTCACAGAGGTCAGCTTCAGGGAAA40300.2874745963050598No Hit
GTGTATTACTGTGCGGGGTCTGTCGCCCGAGACTTGGAGTGGTTATTTGG38180.2723518631991857No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC30790.2196362982688037No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29290.20893625125993054No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT28960.20658224091797844No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27870.19880687342486392No Hit
GTCTCTGGTGGCTCCGTCACCAGTAATAGTTACTACTGGGGCTGGATCCG25930.1849681459600546No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC24490.1746961008315363No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21320.15208333481945097No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18860.13453525772489894No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18180.12968456974754308No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA17980.12825789681302666No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17870.12747322669904262No Hit
GTATTACTGTGCGGGGTCTGTCGCCCGAGACTTGGAGTGGTTATTTGGGCCCCATAGCTGGTTCGACCCCTGGGG17770.1267598902317844No Hit
CAGTAATACACAGCCGTGTCTGCGGCACTCACAGAGGTCAGCTTCAGGGA17570.125333217297268No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG17420.12426321259638066No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17120.12212320319460604No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15840.11299249641370092No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15590.11120915524555537No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15520.11070981971847463No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15440.11013915054466805No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15280.10899781219705493No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT14840.10585913174111879No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14780.10543112986076385No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT14760.10528846256731221No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14720.10500312798040892No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA14330.1022211157581019No Hit
CACCATATCCGTGCACACGTCCGAGAACCAGTTTTCCCTGAAGCTGACCT14170.10107977741048875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGAT11600.045.19489770
GTATCAA25800.037.916461
GTGGTAT5050.036.02361
TATCAAC29050.033.433432
ATCAACG31800.030.217333
AACGCAG31800.030.217336
TCAACGC31900.030.0150224
CAACGCA32150.029.7816265
AGAGTAC34150.027.63548711
TGTTAGA4500.026.9944670
GCACGGA12450.026.96075469
CAGAGTA35450.026.62300510
CGCAGAG35550.026.5481198
GCAGAGT36450.025.9867639
TGTATTA13500.024.9133992
TTCGGGG12950.024.68503870
ACGCAGA39250.024.3078067
GTACTAA1001.6270196E-524.0270611
GTGTATT14600.023.5098461
GTATTAC14550.023.1146983
Produced by FastQC (version 0.11.5)