FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245809
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGAACTGTGGCTGCACCAT78870.633082599339064No Hit
CTATAATACTGTTGACAGTAATAAGTTGCAGAATCTTCAGGCTGCAGGCT71020.5700713351725666No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54840.4401958887758878No Hit
ATAGTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGA46890.3763819333461229No Hit
GGGTACTATAATACTGTTGACAGTAATAAGTTGCAGAATCTTCAGGCTGCAGGCTGATGATGGTGAGAGTGTACT39310.3155379355904477No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38710.3107217880108427No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35100.2817446334068866No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30340.2435365292753544No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30130.2418508776224927No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30090.2415298011171857No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCAGCATCACTT29960.24048630247493796No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29290.23510827101104584No Hit
GTCTGCATCTGTGGGAGACAGAGTCAGCATCACTTGCCGGGCGAGTCAGGACATTAGGAATTCTTTAGCCTGGTA28980.23261992809491663No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG28640.2298907777998072No Hit
AGTCAGGACATTAGGAATTCTTTAGCCTGGTACCAGCAGAAACCAGGGAA26620.213676414281804No Hit
TGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC26340.21142887874465507No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACAT26320.21126834049200158No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26050.20910107408117937No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25790.20701407679668393No Hit
GAGTACACTCTCACCATCATCAGCCTGCAGCCTGAAGATTCTGCAACTTATTACTGTCAACAGTATTATAGTACC25480.20452573388055473No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC24920.2000306628062568No Hit
GTGCAGCCACAGTTCGTTCGATCTCCACCTTGGTCCCTCCGCCGAAAGTG24900.1998701245536033No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23430.18807056298357133No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22990.1845387214251944No Hit
GAGTGTACTCCGTCACAGATCCACTGCCACTGAACCTGGATGGGACCCCA22250.178598806077015No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21830.1752275027712916No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20230.16238444255901185No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19770.15869206274798142No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19690.15804990973736743No Hit
GTAATAGACGGCCGTGTCTTCGACTCTCAGGCTGTCCATTTGCAGATACA19580.1571669493477732No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19450.1561234507055255No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18510.14857815283081116No Hit
GTGGGAGACAGAGTCAGCATCACTTGCCGGGCGAGTCAGGACATTAGGAATTCTTTAGCCTGGTACCAGCAGAAA18260.14657142467264245No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC18240.14641088641998895No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17900.1436817361248795No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17890.14360146699855275No Hit
GTACTATAATACTGTTGACAGTAATAAGTTGCAGAATCTTCAGGCTGCAG17660.14175527709303753No Hit
GTCTATTACTGTGCGAGAGGAGGCGCCTACAGTAACTACCCCTACTACTA17620.14143420058773054No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17530.1407117784507898No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16900.13565482349220467No Hit
GTAATAAGTTGCAGAATCTTCAGGCTGCAGGCTGATGATGGTGAGAGTGTACTCCGTCACAGATCCACTGCCA16850.13525347786057093No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16740.1343705174709767No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16420.13180190542852074No Hit
GTACCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCAGCATCA16270.13059786853361954No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16100.12923329338606482No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15740.12634360483830187No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15620.1253803753223809No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15490.12433687668013316No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15260.12249068677461794No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15150.1216077263850237No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15070.12096557337440972No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14990.12032342036379573No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14750.11839696133195378No Hit
GAGTCAGCATCACTTGCCGGGCGAGTCAGGACATTAGGAATTCTTTAGCCTGGTACCAGCAGAAACCAGGGAAAG14600.11719292443705255No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG14340.1151059271525571No Hit
ATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGGGTACTATAATACTG14260.1144637741419431No Hit
TTATAGTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGAACTGTGGCTGCACCATCTGTCTT14230.11422296676296287No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14210.11406242851030937No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14090.11309919899438839No Hit
GCCACAGTTCGTTCGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGG13950.1119754312258139No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13870.11133327821519992No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13700.10996870306764521No Hit
CCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCAGCATCACTTGCCG13640.10948708830968472No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13420.10772116753049625No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATCTCCACCTTGGTCC13350.107159283646209No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTTAGTACCTATTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAAGGG13340.10707901451988226No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA13300.10675793801457527No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13230.10619605413028804No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12940.10386824946681233No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC12800.10274448169823784No Hit
GACCAAGGTGGAGATCGAACGAACTGTGGCTGCACCATCTGTCTTCATCT12730.10218259781395062No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12710.10202205956129712No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12670.10170098305599011No Hit
ATAATACTGTTGACAGTAATAAGTTGCAGAATCTTCAGGCTGCAGGCTGA12560.10081802266639589No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)