FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245809
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGAACTGTGGCTGCACCAT74080.5946336878285515No Hit
CTATAATACTGTTGACAGTAATAAGTTGCAGAATCTTCAGGCTGCAGGCT64040.5140434849964962No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55630.44653714975570086No Hit
ATAGTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGA40330.323725386475776No Hit
GGGTACTATAATACTGTTGACAGTAATAAGTTGCAGAATCTTCAGGCTGCAGGCTGATGATGGTGAGAGTGTACT37030.297236574787949No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33270.2670553832890917No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32750.26288138872010075No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31810.2553360908453864No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31340.2515634419080292No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29980.24064684072759146No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCAGCATCACTT29180.23422531062145163No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28540.22908808653653973No Hit
GTCTGCATCTGTGGGAGACAGAGTCAGCATCACTTGCCGGGCGAGTCAGGACATTAGGAATTCTTTAGCCTGGTA27550.22114144303019162No Hit
TGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25980.20853919019689213No Hit
GTGGGAGACAGAGTCAGCATCACTTGCCGGGCGAGTCAGGACATTAGGAATTCTTTAGCCTGGTACCAGCAGAAA25680.20613111640708967No Hit
GTGCAGCCACAGTTCGTTCGATCTCCACCTTGGTCCCTCCGCCGAAAGTG25630.20572977077545596No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24730.1985055494060486No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG24690.1981844729007416No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23810.19112078978398775No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACAT23790.19096025153133425No Hit
GAGTGTACTCCGTCACAGATCCACTGCCACTGAACCTGGATGGGACCCCA23640.18975621463643302No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23230.18646518045703636No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC22970.1843781831725409No Hit
AGTCAGGACATTAGGAATTCTTTAGCCTGGTACCAGCAGAAACCAGGGAA22930.1840571066672339No Hit
GAGTACACTCTCACCATCATCAGCCTGCAGCCTGAAGATTCTGCAACTTATTACTGTCAACAGTATTATAGTACC22780.18285306977233268No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22390.17972257384558948No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21380.1716153920865879No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21020.168725703538825No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20490.16447143984350732No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19970.1602974452745164No Hit
GTAATAGACGGCCGTGTCTTCGACTCTCAGGCTGTCCATTTGCAGATACA19890.15965529226390243No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19770.15869206274798142No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19590.15724721847409998No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19560.1570064110951197No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19410.1558023742002185No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18270.14665169379896917No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC18270.14665169379896917No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18100.14528711865141444No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17700.14207635359834453No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17570.1410328549560968No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16670.13380863358668946No Hit
GTCTATTACTGTGCGAGAGGAGGCGCCTACAGTAACTACCCCTACTACTA16540.13276513494444173No Hit
GTACTATAATACTGTTGACAGTAATAAGTTGCAGAATCTTCAGGCTGCAG16530.13268486581811498No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16380.13148082892321375No Hit
GTAATAAGTTGCAGAATCTTCAGGCTGCAGGCTGATGATGGTGAGAGTGTACTCCGTCACAGATCCACTGCCA16200.13003598464933228No Hit
GTACCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCAGCATCA14980.12024315123746898No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14940.11992207473216199No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14880.1194404599742015No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14870.11936019084787476No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14650.11759427006868628No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14470.11614942579480482No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14390.11550727278419083No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13980.11221623860479416No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13910.11165435472050693No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13790.11069112520458593No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13380.10740009102518926No Hit
GAGTCAGCATCACTTGCCGGGCGAGTCAGGACATTAGGAATTCTTTAGCCTGGTACCAGCAGAAACCAGGGAAAG13360.10723955277253576No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13270.10651713063559504No Hit
GCCACAGTTCGTTCGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGG13230.10619605413028804No Hit
TTATAGTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGAACTGTGGCTGCACCATCTGTCTT13220.10611578500396128No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA13080.10499201723538681No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC13070.10491174810906005No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13020.10451040247742632No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13020.10451040247742632No Hit
CTGCTGCTCTGGCTCCCAGGTACCAGATGTGACATCCAGATGACCCAGTC12930.10378798034048559No Hit
ATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGGGTACTATAATACTG12830.1029852890772181No Hit
GTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGTGACGGAGTACACT12530.10057721528741566No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTTAGTACCTATTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAAGGG12470.10009560052945515No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGC306.4684445E-445.7715071
GTGGTAT3550.041.5811581
GTATCAA23250.040.6037561
CGAATAT350.001364711239.32903730
GGTATCA8950.038.356011
TGGTATC3900.037.84042
TATCAAC26450.035.6827962
ATCAACG27700.034.0698283
TCAACGC28050.033.644714
AACGCAG28350.033.5361636
CAACGCA28150.033.5292325
CGCAGAG30200.031.3719258
AGAGTAC30100.030.67784311
CGTTATA450.004699726630.5634227
GGTATAC450.004736820230.5143381
CAGAGTA30450.030.32522410
GCAGAGT31000.030.1193969
TGTTAGA8250.029.32703670
ACGCAGA33000.028.9181087
TCTGGAG12750.027.69516870
Produced by FastQC (version 0.11.5)