FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences809079
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12750.15758658919586344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGTG504.0099385E-641.142145
GGTATCA14200.039.5993421
GTATCAA33550.038.9372181
GTGGTAT3750.036.573051
TGGTATC3850.036.5114252
TATCAAC37650.034.5127752
GTCTATA652.4209077E-531.6497571
AACGCAG41550.031.194656
CAACGCA42250.030.6740255
ATCAACG42800.030.5201643
ATAGAGT450.004760998330.4813148
CCACTAG450.00476387830.4775451
TCAACGC42750.030.4756624
CGCAGAG45400.028.7021168
CAGAGTA44750.028.65924610
AGAGTAC44800.028.62725811
ACGCAGA46250.028.1746187
ATAGTGT500.00796817527.4314846
TATAAGA3900.027.2522752
GCAGAGT48800.026.8429189
Produced by FastQC (version 0.11.5)