FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038392
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGAACTGTGGCTGCACCAT46450.4473262505874468No Hit
ATAGTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGA40920.3940708325950123No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33610.32367352599018484No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31430.3026795275772541No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29610.2851524279848073No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28140.27099592446783105No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC27320.26309909937672865No Hit
CTATAATACTGTTGACAGTAATAAGTTGCAGAATCTTCAGGCTGCAGGCT27070.2606915307513925No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT26460.25481706330557247No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26310.2533725221303708No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25110.24181619272875757No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC23420.22554102882148555No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21540.20743611275895807No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21420.20628047981879677No Hit
GAGTGTACTCCGTCACAGATCCACTGCCACTGAACCTGGATGGGACCCCA21360.20570266334871606No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20920.2014653425681246No Hit
GTCTATTACTGTGCGAGAGGAGGCGCCTACAGTAACTACCCCTACTACTA20770.20002080139292291No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20310.1955908751223045No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20220.1947241504171835No Hit
GTAATAGACGGCCGTGTCTTCGACTCTCAGGCTGTCCATTTGCAGATACA19410.18692362807109453No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19280.18567169238591977No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18180.17507839043444093No Hit
GGGTACTATAATACTGTTGACAGTAATAAGTTGCAGAATCTTCAGGCTGCAGGCTGATGATGGTGAGAGTGTACT17760.1710336751438763No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17410.16766307906840577No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17340.16698895985331166No Hit
TGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17240.16602593240317723No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCAGCATCACTT17190.16554441867810998No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16970.16342575828781422No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16860.16236642809266635No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA16570.15957364848727648No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC16470.15861062103714205No Hit
GTCTGCATCTGTGGGAGACAGAGTCAGCATCACTTGCCGGGCGAGTCAGGACATTAGGAATTCTTTAGCCTGGTA16400.15793650182204794No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16230.1562993551568194No Hit
AGTCAGGACATTAGGAATTCTTTAGCCTGGTACCAGCAGAAACCAGGGAA16170.15572153868673874No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16160.15562523594172528No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16110.15514372221665806No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACAT15840.15254354810129508No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15710.1512916124161203No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15300.14734319987056912No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15180.1461875669304078No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15110.14551344771531366No Hit
GTGCAGCCACAGTTCGTTCGATCTCCACCTTGGTCCCTCCGCCGAAAGTG14970.14416520928512547No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14740.14195024614981627No Hit
GAGTACACTCTCACCATCATCAGCCTGCAGCCTGAAGATTCTGCAACTTATTACTGTCAACAGTATTATAGTACC14560.14021679673957427No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14540.14002419124954738No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT14480.13944637477946673No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14180.1365572924290634No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG14170.13646098968404996No Hit
CTCCAGCCCTTTCCCTGGAGCCTGGCGGACCCAACTCATCCAATAGGTAC14170.13646098968404996No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14030.13511275125386174No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13950.1343423292937542No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13860.1334756045886332No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13810.13299409086356598No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTTAGTACCTATTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAAGGG13770.1326088798835122No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13270.12779374263284002No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG13020.1253861740075039No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12910.12432684381235602No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12780.12307490812718126No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12740.12268969714712749No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA12590.12124515597192581No Hit
TTATAGTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGAACTGTGGCTGCACCATCTGTCTT12470.1200895230317645No Hit
GTAATAAGTTGCAGAATCTTCAGGCTGCAGGCTGATGATGGTGAGAGTGTACTCCGTCACAGATCCACTGCCA12180.11729674342637463No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA11960.11517808303607886No Hit
GTGGGAGACAGAGTCAGCATCACTTGCCGGGCGAGTCAGGACATTAGGAATTCTTTAGCCTGGTACCAGCAGAAA11890.11450396382098477No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT11840.11402245009591754No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11840.11402245009591754No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG11660.11228900068567554No Hit
GAGCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGGTACCAGGCTAAAG11650.1121926979406621No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCCAGATGGAAGTGAGAAATACTA11600.11171118421559488No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG11600.11171118421559488No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11590.11161488147058143No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG11580.111518578725568No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC11500.11074815676546046No Hit
GACCAAGGTGGAGATCGAACGAACTGTGGCTGCACCATCTGTCTTCATCT11430.11007403755036634No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11320.10901470735521845No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT11210.10795537716007056No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC11170.10757016618001679No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG11130.10718495519996302No Hit
CTCCAGAGACAACGCCAAGAACTCTCTGTATCTGCAAATGGACAGCCTGAGAGTCGAAGACACGGCCGTCTATTA11110.10699234970993614No Hit
GTGTACTCCGTCACAGATCCACTGCCACTGAACCTGGATGGGACCCCACT11090.10679974421990925No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10910.10506629480966725No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC10870.10468108382961348No Hit
GGATGGGACCCCACTTTCCAATTTGGAGACAACAGAGAGCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGGTA10780.10381435912449248No Hit
TGGTGAGAGTGTACTCCGTCACAGATCCACTGCCACTGAACCTGGATGGGACCCCACTTTCCAATTTGGAGACAA10740.10342914814443871No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10460.1007326712840623No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10410.10025115755899507No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT10390.10005855206896817No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAT200.007182148352.06902744
AGACGCG250.00864745349.67923469
GTGGTAT1850.042.7094541
CGCTAAT1300.042.3891332
CGTATGC1400.042.0692341
CTAATCT1150.041.95268634
GCTAATC1250.041.33938233
CTTATAC1450.040.276231
ATACACA1450.040.274294
TGTATTA350.001376620639.2589724
TATACAC1500.038.9318123
TACACAT1700.038.3929675
CTCGCTA1550.037.7450230
CGAGACA1550.037.68865223
TTATACA1550.037.675952
CTCGTAT1400.037.0515239
CTCTTAT655.635593E-736.9958151
TCGTATG1500.036.91906740
TAATCTC1350.035.75647435
ACACTCG1650.035.431827
Produced by FastQC (version 0.11.5)