FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038392
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGAACTGTGGCTGCACCAT43390.41785761061333293No Hit
ATAGTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGA37270.35892033066510526No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34130.32868126873088394No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33070.3184731777594589No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT31110.29959783973682386No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT30400.2927603448408693No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC26210.2524094946802364No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25890.24932780683980618No Hit
CTATAATACTGTTGACAGTAATAAGTTGCAGAATCTTCAGGCTGCAGGCT24770.23854189939830048No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23750.22871901940692915No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22970.22120740529588054No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC22660.21822202020046377No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22490.21658487353523526No Hit
GAGTGTACTCCGTCACAGATCCACTGCCACTGAACCTGGATGGGACCCCA22130.21311797471475127No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21670.20868804844413283No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21000.20223576452823214No Hit
GTAATAGACGGCCGTGTCTTCGACTCTCAGGCTGTCCATTTGCAGATACA20380.1962649943373986No Hit
GTCTATTACTGTGCGAGAGGAGGCGCCTACAGTAACTACCCCTACTACTA18960.18259000454548957No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18620.17931571121503248No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18480.17796747278484426No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17760.1710336751438763No Hit
GTCTGCATCTGTGGGAGACAGAGTCAGCATCACTTGCCGGGCGAGTCAGGACATTAGGAATTCTTTAGCCTGGTA16950.16323315279778736No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16840.16217382260263946No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16680.16063297868242438No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCAGCATCACTT16670.16053667593741092No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16530.1591884375072227No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16400.15793650182204794No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16220.15620305241180596No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA16060.15466220849159085No Hit
TGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15940.15350657555142952No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15940.15350657555142952No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15770.15186942888620097No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15690.15109900692609343No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15560.14984707124091864No Hit
GGGTACTATAATACTGTTGACAGTAATAAGTTGCAGAATCTTCAGGCTGCAGGCTGATGATGGTGAGAGTGTACT15490.14917295202582453No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15490.14917295202582453No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15420.14849883281073042No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15300.14734319987056912No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACAT15210.14647647516544812No Hit
CTCCAGCCCTTTCCCTGGAGCCTGGCGGACCCAACTCATCCAATAGGTAC15080.14522453948027336No Hit
GTGGGAGACAGAGTCAGCATCACTTGCCGGGCGAGTCAGGACATTAGGAATTCTTTAGCCTGGTACCAGCAGAAA15030.14474302575520612No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14810.14262436536491035No Hit
AGTCAGGACATTAGGAATTCTTTAGCCTGGTACCAGCAGAAACCAGGGAA14750.14204654889482968No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14720.14175764065978935No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14680.1413724296797356No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14660.1411798241897087No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14630.14089091595466838No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT14540.14002419124954738No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14300.13771292536922472No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTTAGTACCTATTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAAGGG14290.1376166226242113No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14280.13752031987919783No Hit
GTGCAGCCACAGTTCGTTCGATCTCCACCTTGGTCCCTCCGCCGAAAGTG14260.13732771438917096No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14140.13617208144900964No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14060.13540165948890207No Hit
GAGTACACTCTCACCATCATCAGCCTGCAGCCTGAAGATTCTGCAACTTATTACTGTCAACAGTATTATAGTACC13960.13443863203876763No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT13860.1334756045886332No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13310.12817895361289378No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13230.12740853165278623No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13180.12692701792771902No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCCAGATGGAAGTGAGAAATACTA13030.12548247675251734No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12230.11777825715144186No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12190.11739304617138807No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG12110.11662262421128052No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA12040.1159485049961864No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12040.1159485049961864No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12030.11585220225117296No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11980.11537068852610574No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA11930.11488917480103854No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA11910.11469656931101165No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT11650.1121926979406621No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG11620.11190378970562177No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11310.10891840461020501No Hit
GTAATAAGTTGCAGAATCTTCAGGCTGCAGGCTGATGATGGTGAGAGTGTACTCCGTCACAGATCCACTGCCA11210.10795537716007056No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11190.1077627716700437No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC11180.10766646892503023No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11170.10757016618001679No Hit
GAGCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGGTACCAGGCTAAAG11120.10708865245494957No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA11090.10679974421990925No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11080.1067034414748958No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG11070.10660713872988235No Hit
TGGTGAGAGTGTACTCCGTCACAGATCCACTGCCACTGAACCTGGATGGGACCCCACTTTCCAATTTGGAGACAA11060.10651083598486892No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG11040.10631823049484203No Hit
CTCCAGAGACAACGCCAAGAACTCTCTGTATCTGCAAATGGACAGCCTGAGAGTCGAAGACACGGCCGTCTATTA10840.10439217559457316No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10820.10419957010454625No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGTCC10800.10400696461451937No Hit
GGCTGATGATGGTGAGAGTGTACTCCGTCACAGATCCACTGCCACTGAAC10780.10381435912449248No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10620.10227351520427738No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC10540.10150309324416984No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC10450.10063636853904884No Hit
TTATAGTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGAACTGTGGCTGCACCATCTGTCTT10430.10044376304902194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACA400.00938303248.6609870
GTGGTAT2550.041.7360651
TGGTATC2650.040.1553152
GTATCAA13350.037.288651
GGTATCA6000.035.4756551
CTTAATG400.00250778834.74462546
TATCAAC15250.032.6381382
CTATACT553.2536013E-431.36889536
ATCAACG16100.030.912033
TAGGCAA1053.5545934E-430.89585970
TTGGACC450.00474025430.5093444
ATTGGCG703.433162E-530.0728258
TCAACGC16700.029.801424
AACGCAG17100.029.5100126
GTGTATG703.9816317E-529.426811
TACTCAA605.3538923E-428.81200839
CAACGCA17300.028.7678435
TTCGTGT500.00648007428.61876166
CATGCTA605.5874855E-428.602514
AAATACC605.5874855E-428.602514
Produced by FastQC (version 0.11.5)