FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763184
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25020.3278370615736179No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23460.30739638147550263No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22350.2928520514056899No Hit
GTCCCAGACCCACTGCCTCTGAACCTGGCTGGGATACCTTTGCCCCTGGT21370.2800111113440534No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCT21250.27843875133650603No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20070.2629772112622906No Hit
CCATAGATGAGGAGCCTGGGAGCCTGCCCAGGTTTCTGCTGGTACCAGGC18420.24135726115851486No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17560.23008868110442568No Hit
GTAATAGACTGCAAAGTCTTCAGACTGCAGGCCACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCT16860.22091658106039957No Hit
GTATTATAAGTGGGTCACTTTCGGCCCTGGGACCAAAGTGTATATCAAACGAACTGTGGCTGCACCATCTGTCTT16830.22052349105851277No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCGGGGCCAGTGAGAGTGTCAGCAG15680.2054550409861842No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15490.20296547097423426No Hit
GTCTTCAGACTGCAGGCCACTGATGGTGAGAGTGAAGTCTGTCCCAGACC15220.19942766095725276No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14570.1909107109163714No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14440.18920732090819514No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCTCTGGAGAAATAGTG14410.1888142309063083No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14100.18475230088681105No Hit
CTTATAATACTGCTGACAGTAATAGACTGCAAAGTCTTCAGACTGCAGGCCACTGATGGTGAGAGTGAAGT14070.1843592108849242No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14000.18344200088052162No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13830.18121449086982955No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13380.17531814084152708No Hit
CAGTAATAGACTGCAAAGTCTTCAGACTGCAGGCCACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCT13290.17413887083586657No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13020.1706010608188851No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12900.16902870081133775No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGTGGCCTGCAGTCTGAAGACT12720.1666701608000168No Hit
GTGTATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC12550.1644426507893247No Hit
GCCTGGGAGCCTGCCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTGCTG12480.16352544078492212No Hit
GTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCGGGGCCAGTGAGAGTGTCAGCAGCAACTTAGCCTGGTA12010.15736703075536174No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCTCTGAACCTGGCTGGGATACCT11720.15356716073712237No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11430.149767290718883No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11410.1495052307176251No Hit
GTCTATTACTGTCAGCAGTATTATAAGTGGGTCACTTTCGGCCCTGGGAC11110.1455743306987568No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11090.1453122706974989No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10380.1360091406528439No Hit
CCTTTGCCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGCCC10370.13587811065221492No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10320.13522296064907022No Hit
CTTTCGGCCCTGGGACCAAAGTGTATATCAAACGAACTGTGGCTGCACCA10050.13168515063208874No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT9950.1303748506257993No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9890.12958867062202561No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9620.12605086060504414No Hit
CTATTACTGTCAGCAGTATTATAAGTGGGTCACTTTCGGCCCTGGGACCAAAGTGTATATCAAACGAACTGTGG9570.1253957106018994No Hit
CATCTATGGTGCATCCACCAGGGGCAAAGGTATCCCAGCCAGGTTCAGAGGCAGTGGGTCTGGGACAGACTTCA9510.12460953059812575No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9400.12316820059120735No Hit
CTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCTCTGAACCTGGC9390.12303717059057842No Hit
CTGCTACTCTGGCTCCCAGATACCTCTGGAGAAATAGTGCTGACGCAGTC9360.12264408058869158No Hit
GGTTTCTGCTGGTACCAGGCTAAGTTGCTGCTGACACTCTCACTGGCCCCGCAGGAGAGGGTGACTCTTTCCC9330.12225099058680476No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9210.12067863057925743No Hit
GTCTGAAGACTTTGCAGTCTATTACTGTCAGCAGTATTATAAGTGGGTCA9120.11949936057359693No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9000.1179270005660496No Hit
CTCCTGCGGGGCCAGTGAGAGTGTCAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGGCAGGCTCCCAGGCT8670.11360301054529444No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8180.10718254051447619No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7830.10259649049246317No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT7690.10076207048365794No Hit
CACCATAGATGAGGAGCCTGGGAGCCTGCCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTGCTGACACTCTCA7670.10050001048240004No Hit
GACTTCACTCTCACCATCAGTGGCCTGCAGTCTGAAGACTTTGCAGTCTA7640.10010692048051321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)