FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763184
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25140.32940942158116526No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22380.2932451414075767No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCT21850.28630055137424265No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21810.28577643137172687No Hit
GTCCCAGACCCACTGCCTCTGAACCTGGCTGGGATACCTTTGCCCCTGGT21180.2775215413321034No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19540.25603262122895654No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18880.2473846411874463No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17600.23061280110694146No Hit
GTATTATAAGTGGGTCACTTTCGGCCCTGGGACCAAAGTGTATATCAAACGAACTGTGGCTGCACCATCTGTCTT17470.22890941109876517No Hit
GTCTTCAGACTGCAGGCCACTGATGGTGAGAGTGAAGTCTGTCCCAGACC16590.2173787710434181No Hit
GTAATAGACTGCAAAGTCTTCAGACTGCAGGCCACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCT16220.21253066102014717No Hit
CCATAGATGAGGAGCCTGGGAGCCTGCCCAGGTTTCTGCTGGTACCAGGC16060.21043418101008407No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCGGGGCCAGTGAGAGTGTCAGCAG15760.20650328099121573No Hit
CTTATAATACTGCTGACAGTAATAGACTGCAAAGTCTTCAGACTGCAGGCCACTGATGGTGAGAGTGAAGT15750.20637225099058681No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14860.1947105809346108No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14430.1890762909075662No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14210.18619363089372945No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14170.18566951089121364No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGTGGCCTGCAGTCTGAAGACT14000.18344200088052162No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13640.17872492085787964No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13440.17610432084530073No Hit
GTGTATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC12800.16771840080504832No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCTCTGGAGAAATAGTG12770.1673253108031615No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12570.16470471079058263No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12570.16470471079058263No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12500.16378750078617998No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12450.16313235078303528No Hit
CAGTAATAGACTGCAAAGTCTTCAGACTGCAGGCCACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCT12410.1626082307805195No Hit
GCCTGGGAGCCTGCCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTGCTG12140.159070420763538No Hit
GTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCGGGGCCAGTGAGAGTGTCAGCAGCAACTTAGCCTGGTA11850.15527055074529864No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCTCTGAACCTGGCTGGGATACCT11710.15343613073649343No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11430.149767290718883No Hit
GTCTATTACTGTCAGCAGTATTATAAGTGGGTCACTTTCGGCCCTGGGAC11050.14478815069498313No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11010.14426403069246735No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10730.1405951906748569No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT10660.1396779806704543No Hit
CTGCTACTCTGGCTCCCAGATACCTCTGGAGAAATAGTGCTGACGCAGTC10580.13862974066542275No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10490.13745047065976226No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9930.1301127906245414No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9740.12762322061259146No Hit
CTATTACTGTCAGCAGTATTATAAGTGGGTCACTTTCGGCCCTGGGACCAAAGTGTATATCAAACGAACTGTGG9700.12709910061007568No Hit
GGTTTCTGCTGGTACCAGGCTAAGTTGCTGCTGACACTCTCACTGGCCCCGCAGGAGAGGGTGACTCTTTCCC9590.1256577706031573No Hit
CTTTCGGCCCTGGGACCAAAGTGTATATCAAACGAACTGTGGCTGCACCA9310.12198893058554686No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9300.12185790058491792No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9240.12107172058114427No Hit
CATCTATGGTGCATCCACCAGGGGCAAAGGTATCCCAGCCAGGTTCAGAGGCAGTGGGTCTGGGACAGACTTCA9020.11818906056730749No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8890.11648567055913123No Hit
GTCTGAAGACTTTGCAGTCTATTACTGTCAGCAGTATTATAAGTGGGTCA8880.11635464055850227No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8820.11556846055472861No Hit
CTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCTCTGAACCTGGC8750.114651250550326No Hit
CCTTTGCCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGCCC8650.11334095054403658No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8500.1113755005346024No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8250.1080997505188788No Hit
CTCCTGCGGGGCCAGTGAGAGTGTCAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGGCAGGCTCCCAGGCT8240.10796872051824985No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8230.10783769051762092No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT7790.10207237048994737No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7790.10207237048994737No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT7720.10115516048554477No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA200.007579728551.3629926
ACTCTTA200.007629014651.2788628
GGTATCA4600.042.3663521
GTATCAA11350.039.1607931
ATAAGGC400.00247837434.8270947
TATCAAC13300.033.159932
ATCAACG13650.032.5580063
GTGGTAT1850.031.4181941
CAACGCA14150.031.4075475
TACTATA553.2965367E-431.29750634
AACGCAG14250.031.1871456
TCAACGC14450.030.7554864
AGAGTAC14000.030.5231311
GAGTGGA4900.029.84690570
CGCAGAG14550.029.6042928
CATGCTA704.097169E-529.3022064
TGGTATC1900.028.7900182
GCAGAGT15000.028.7161669
TCTGTAC756.5567336E-527.3487263
TGTTAGA7200.025.85224370
Produced by FastQC (version 0.11.5)