FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219971
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT69590.5704233953102164No Hit
GTCTACAAACTCCTCGGACTTTCGGCGGAGGGACCAGGGTGGAGATCAAA61980.5080448633615061No Hit
AGCCAGGACAGTCTCCACAACTCCTAATCTATTTGGGCTCTTATCGGGCC59280.48591318973975606No Hit
GTTTGTAGACCTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAGT45360.37181211684540044No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45120.36984485696791153No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43410.3558281303408032No Hit
GTCTTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTCCACAACTCCTAATCTATTTGGGCT36230.29697427233926055No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34800.28525268223588923No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34750.284842836428079No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34620.28377723732777255No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34420.2821378540965318No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34240.2806624091884152No Hit
GGCACAGACTTCACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTA34160.28000665589591883No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTC33840.27738364272593363No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33270.2727114005168975No Hit
GTCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCAAGACTATGC31530.25844876640510306No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGACTTCACACTGAA27140.22246430447936874No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTCTTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTC26630.2182838772397049No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25990.2130378508997345No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25900.21230012844567617No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25310.20746394791351597No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25160.20623441049008542No Hit
CTCCTGCATAGTCTTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTCCACAACTCCTAAT24820.20344745899697614No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT24670.20221792157354557No Hit
ATCCAAGACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC23400.19180783805516688No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23380.19164389973204282No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAGTCTGTGCCTGATCCACTGCCA23060.18902088656205762No Hit
CCCCTGGAGAACCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG23020.18869300991580948No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22770.18664378087675856No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG22630.18549621261489002No Hit
GTCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCC21940.17984034046710948No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAGCCCAAATAGATTAGGAGTTGTGGAGACTGTCCTGGCTTCTGCAGGTA21630.17729929645868633No Hit
GTTCAGTGGCAGTGGATCAGGCACAGACTTCACACTGAAAATCAGCAGAG21630.17729929645868633No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC21610.17713535813556222No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20430.16746299707124185No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGACTCAGTCTCCAGTCTCCCTGCCCGTCACCC19430.15926608091503816No Hit
GACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT19310.1582824509762937No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19210.15746275936067333No Hit
GTTTATTACTGCATGCAAGGTCTACAAACTCCTCGGACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA18960.1554135303216224No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18820.1542659620597539No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18600.15246264050538907No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18570.15221673302070296No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18560.1521347638591409No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGTTCTCCAGGGGTGACGGGCAGGGAGACTGGAGA18560.1521347638591409No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG18500.1516429488897687No Hit
TTATTACTGCATGCAAGGTCTACAAACTCCTCGGACTTTCGGCGGAGGGA18440.15115113392039647No Hit
GTCTCCACAACTCCTAATCTATTTGGGCTCTTATCGGGCCTCCGGGGTCC18050.14795433661947702No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18030.14779039829635293No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGACTCAGTCTCCAGT17940.14705267584229462No Hit
GTCTCCAGTCTCCCTGCCCGTCACCCCTGGAGAACCGGCCTCCATCTCCT17330.14205255698701036No Hit
CAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAGTCTGTGCCTGATCCACTGCCA17300.14180664950232424No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17250.14139680369451404No Hit
CTTATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATCAGGC17050.1397574204632733No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGACTCAGTCTCCAGTCTCC17030.13959348214014924No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16930.13877379052452887No Hit
GTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTACAAAC16840.13803606807047053No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16740.13721637645485016No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGA16510.1353310857389233No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16380.13426548663861684No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16010.13123262766082144No Hit
ACCCTGGTCCCTCCGCCGAAAGTCCGAGGAGTTTGTAGACCTTGCATGCAGTAATAAACTCCAACATCCTCAGC15820.12967521359114276No Hit
GACTTCACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTACAAACT15530.12729810790584367No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15140.12410131060492421No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG14760.12098648246556681No Hit
GAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTACAAACTCCTCGGACTTTCGGCGGAGGGA14680.12033072917307051No Hit
GTACCTGCAGAAGCCAGGACAGTCTCCACAACTCCTAATCTATTTGGGCTCTTATCGGGCCTCCGGGGTCCCTGA14370.11778968516464736No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGACTCAGTCTC14370.11778968516464736No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14200.11639620941809273No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13910.11401910373279366No Hit
TCTCCACCCTGGTCCCTCCGCCGAAAGTCCGAGGAGTTTGTAGACCTTGCATGCAGTAATAAACTCCAACATCCT13790.1130354737940492No Hit
CTCCACAACTCCTAATCTATTTGGGCTCTTATCGGGCCTCCGGGGTCCCT13640.11180593637061866No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13560.11115018307812236No Hit
AGTCAGAGCCTCCTGCATAGTCTTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTCCACAA13530.11090427559343624No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13400.10983867649312976No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13390.10975670733156771No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13290.10893701571594734No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC13120.10754353996939271No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA12870.10549431093034178No Hit
CTCCTAATCTATTTGGGCTCTTATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGA12820.10508446512253161No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12780.10475658847628344No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12680.10393689686066308No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT12600.10328114356816678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11200.040.444761
GTATCAA27600.039.663221
AATATAG350.001383877139.2175525
GTGGTAT3250.036.9565161
CTTATAC2550.033.6438831
TTATACA2450.033.6150442
AATGGTA2253.6379788E-1233.58629670
TATCAAC33050.033.225162
AACGCAG33950.032.142226
ATCAACG34350.031.668033
CAACGCA34550.031.3853915
TCAACGC34850.031.2136824
AGAGTAC35350.031.05576911
TGGTATC4000.030.8838212
CAGAGTA36200.030.32655710
CGCAGAG36550.030.0386168
TATACTA703.560452E-529.913550
TATACAC2900.029.5822053
GTACGGT703.9948187E-529.4131643
ACGCAGA37450.029.2263097
Produced by FastQC (version 0.11.5)