FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences809645
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA150.002229678769.9601957
GTATCAA21850.037.5347481
GTGGTAT3200.036.6131321
GGTATCA10200.035.810771
GTATAGG501.873848E-434.459421
TATCAAC25550.032.236052
TGGTATC4150.029.8943562
AACGCAG28200.029.084576
ATCAACG28350.028.9306853
TCAACGC28700.028.5778714
AGAGTAC27900.028.52915611
CAACGCA28700.028.4577965
CAGAGTA28250.028.41964110
TACACTT500.00777578627.569245
ATACACT500.00777578627.569244
ACGCAGA30300.027.1825457
TATACAC906.962873E-626.8034293
GCAGAGT30600.026.6875119
CGCAGAG31200.025.9566158
TCGGTTT1250.001321236624.67908970
Produced by FastQC (version 0.11.5)