FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655574
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATACA20210.30827946196768025No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17970.2741109317941224No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15210.23201042140170292No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA12710.1938759011187143No Hit
GTGTATTACTGTGCACGAGCAGTAGGAGTAGCTCGTCCGTTCTCCCTCCA12680.19341828687531842No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12520.19097767757720716No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12000.1830456973583455No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11830.1804525499791023No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11450.17465610289608802No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11390.17374087440929628No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11040.16840204156967786No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11020.16809696540741395No Hit
CTCCTGCAGGGCCAGTCAGGGTGTTAGCAGCACCTACTTAGCCTGGTACC10950.16702919883949027No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT10470.15970737094515647No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10060.15345330961874631No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9910.151165238401767No Hit
GTCTATTACTGTCAGCGCTTTGGTGGGTCACCTCCGTACACTTTTGGCCA9890.1508601622395031No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATA9890.1508601622395031No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9770.14902970526591966No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9690.14780940061686398No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8750.13347082099046026No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8690.13255559250366855No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8680.13240305442253658No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8560.13057259744895314No Hit
CTATTACTGTCAGCGCTTTGGTGGGTCACCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA8490.12950483088102943No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGGGTGTTAGCAG8470.12919975471876555No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCA8440.12874214047536967No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA8400.12813198815084184No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGGTGACCCACCAAAGCG8090.12340330763575126No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA7850.11974239368858436No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT7830.11943731752632045No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7820.11928477944518848No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7750.11821701287726481No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7740.11806447479613286No Hit
GATCAGGACTGAACACACAGGACTCACCATGGAGTTGGGGCTGAGCTGGG7700.11745432247160503No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGGGTGTTAGCAGCA7640.1165390939848133No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7500.11440356084896595No Hit
GTAATAGACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCG7460.11379340852443812No Hit
CTCCTGTGCAGCCTCTGGATTCACCCTCAGAAACTTCGACATACACTGGGTCCGCCAAGGTACAGGGAAAGGT7370.11242056579425053No Hit
GTACAGGGAAAGGTCTGGAGTGGGTCTCAAGTAGTGATACTTCTGGTGAC7350.11211548963198663No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG7140.10891218992821558No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC6960.1061665044678404No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6760.10311574284520131No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTCTGCAGTCTATTACTGTCAGCGCTTTGGTGGGTCA6710.10235305243954153No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC6650.1014378239527498No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA6610.100827671628222No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA6610.100827671628222No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA6590.10052259546595807No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG200.00735875551.7479971
GGTATCA4600.045.7482261
GTATCAA12200.039.588631
TATCAAC13950.034.372392
ATCAACG14550.033.192063
TCAACGC14800.032.6313824
CAACGCA14900.032.4123845
AACGCAG15350.031.9116386
TAGATTG450.00462385730.663145
AGAGTAC16250.029.71285811
CGCAGAG16550.029.3871298
TGGTATC1900.029.049292
GTTTAAC605.3439144E-428.81924833
CAGAGTA17050.028.5209810
GCAGAGT17250.028.3902369
ACGCAGA17300.028.113127
TAACGGC500.007608101227.69189336
GTGGTAT2000.027.598931
GCCGTTA500.00774551927.59051115
CCGTTAA500.00774551927.59051116
Produced by FastQC (version 0.11.5)