FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004038
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13170.13117033419053858No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTA150.002104129470.9912367
CTAGTCC200.00738682251.700213
CTTAAGT306.341077E-445.9557463
GTATCAA25900.042.4556241
GTGGTAT3200.042.010611
GGTATCA9850.040.5943951
GTATAAG1600.038.7790221
TATCAAC28450.038.5252882
CGCTAGA459.7798504E-538.48812539
TGGTATC3600.038.296452
TATAAGA1650.037.600152
ATAAGAG1700.036.4942673
CTATCCT400.002598920434.49257325
ATCTAGG400.002607188434.470241
TACACAG601.3507963E-534.468525
TAAGAGA1750.033.4820444
CAGAGTA32150.033.4516810
AGAGTAC32450.033.24864611
AACGCAG34100.032.5479936
GCAGAGT34100.032.4485249
Produced by FastQC (version 0.11.5)