FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523050
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63400.4162699845704343No Hit
GTATGGTACCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT55760.3661074816979088No Hit
GTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC51190.33610190079117563No Hit
GTATTACTGTCAGCAGTATGGTACCTCACCGTACACTTTTGGCCAGGGGA48910.32113193920094546No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA42280.2776008666819868No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC41740.2740553494632481No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41640.27339877220051867No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC41270.27096943632841997No Hit
GTCTCTGGTGGCTCCATTAGTCCTTTCTACTGGAGCTGGATCCGGCAGTC41080.2697219395292341No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT37690.2474639703227077No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT37240.24450937264042547No Hit
CTCCTGCAGGGCCAGTCAGACTATAAGCAGCATCTCCTTAGCCTGGTACC36530.2398476740750468No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36140.23728702275040217No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36080.23689307639276452No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT35120.23058993467056235No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34360.225599947473819No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC32780.21522602672269459No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31500.2068218377597584No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAATAGCCACCCTCTCCTGC31480.2066905223072125No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAATAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATAAGCAG31400.206165260497029No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT29550.19401858113653525No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG29080.1909326680017071No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28710.18850333212960835No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT28140.18476084173205082No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27520.1806900627031286No Hit
GTCTCAGATACCACCGGAGAACTTGTGTTGACGCAGTCTCCAGGCACCCT27270.1790486195463051No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27130.17812941137848398No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG25790.16933127605791012No Hit
GTGTATTACTGTCAGCAGTATGGTACCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA25600.16808377925872428No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG25230.16565444338662552No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA25140.16506352385016906No Hit
CACCTGCACTGTCTCTGGTGGCTCCATTAGTCCTTTCTACTGGAGCTGGATCCGGCAGTCCCCAGAGAAGGGT24990.16407865795607499No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC24490.16079577164242803No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24360.1599422212008798No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24270.15935130166442338No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC23680.15547749581431994No Hit
GACTATAAGCAGCATCTCCTTAGCCTGGTACCAGCACAAACCTGGCCAGG23470.15409868356258824No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23460.15403302583631528No Hit
GTGAGGTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT23340.15324513312104002No Hit
GTCTTTGTCTCCAGGGGAAATAGCCACCCTCTCCTGCAGGGCCAGTCAGA23160.1520632940481271No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22870.15015921998621187No Hit
GTACCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA22170.14556317914710615No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21950.1441187091691015No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT21910.14385607826400973No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21810.14319950100128032No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGT21720.14260858146482389No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA21600.1418206887495486No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21530.14136108466563801No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC21440.14077016512918158No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC21390.1404418764978169No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT21260.13958832605626867No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT20990.1378155674468993No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20820.13669938610025933No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA20600.13525491612225468No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT20540.13486096976461706No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT20100.13197202980860773No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTACTCATCTTTGGTGCATCCACCAGGGCCACTGGCATCCCAGACAGGTT19880.13052755983060307No Hit
CTGCTACTCTGGCTCCCAGTCTCAGATACCACCGGAGAACTTGTGTTGAC19460.12776993532713962No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19440.12763861987459374No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19260.12645678080168082No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTACCTCA19110.12547191490758675No Hit
GGCCAAAAGTGTACGGTGAGGTACCATACTGCTGACAGTAATACACTGCA19030.12494665309740324No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG18820.12356784084567152No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18720.12291126358294213No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT18110.11890614228029282No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA17890.11746167230228817No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA17820.1170020682183776No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT17800.11687075276583171No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17770.1166737795870129No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA17280.11345655099963887No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17230.1131282623682742No Hit
TCTTTGGTGCATCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT17040.11188076556908834No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17030.11181510784281541No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA16700.1096484028758084No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG16400.10767867108762025No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16180.10623420110961557No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG16160.10610288565706968No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA15970.10485538885788385No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15940.10465841567906503No Hit
TCCTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCTACTCATCTTTGGTGCATCCACCAGGGCCA15780.10360789205869801No Hit
ATAATAGACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA15730.10327960342733332No Hit
CTCCCAGGCTACTCATCTTTGGTGCATCCACCAGGGCCACTGGCATCCCA15710.10314828797478744No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15690.10301697252224155No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC15600.10242605298578511No Hit
CACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGA15540.10203210662814748No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15350.10078460982896162No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15240.10006237483995929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9600.043.4125141
GTATCAA23450.040.538441
TGGATTG18000.034.04744370
TATCAAC27850.033.6280672
ATCAACG29350.031.7900243
AACGCAG29400.031.6230056
TCAACGC29550.031.3418374
CAACGCA29850.031.1442325
GTACGAG450.004660712630.6159861
TATAAGA2600.030.4587942
ATAAGAG2850.030.2012443
GTAGTTT2002.7876013E-829.02992870
AGAGTAC31950.028.99323511
CGCAGAG32450.028.4403768
GCAGAGT32800.028.3468729
GTATAAG3100.027.7766021
GTCTATA500.00779837627.5543921
AATATAC500.00781338527.5435333
GTGTATA1155.732909E-826.9553811
CAGAGTA34950.026.40601310
Produced by FastQC (version 0.11.5)