FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192605
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATTT100.0096646575130.6725970
ACTACTA200.007428498551.6107523
TACTACT200.007428498551.6107522
GTATCAA5150.041.422221
GTACCGA350.001362960939.3224751
TATAAGA350.001362960939.3224752
TATCAAC5950.037.5876582
TGGTATC952.237357E-1036.218072
GGTATCA2150.035.2073331
ATAAGAG400.002625453534.407173
GTGGTAT1003.8744474E-1034.4071661
CAACGCA7350.030.8962335
TAAGAGA450.004674444430.5841484
AACGCAG7450.030.4815146
AGAGTAC7350.030.42020811
CAGAGTA7450.030.0118810
GCAGAGT7550.029.6143769
CGCAGAG7700.029.491868
TCAACGC7850.028.4900134
ATCAACG8050.028.2096043
Produced by FastQC (version 0.11.5)