FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192605
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3510.1822382596505802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTAT150.002317967869.258140
AATCCGA200.007347346351.75429533
CGGTATT200.007400436351.6601723
GTGTATA200.00741565751.633341
ACACACT200.00742327651.6199386
TGTATAA200.00742327651.6199382
CACAGAT200.00742327651.6199387
GGTATCA2400.048.7648281
GTATCAA5950.040.496741
TATCAAC6550.036.777562
GTGGTAT601.3575869E-534.422231
TACTGCT501.88333E-434.4132927
TGGTATC501.88333E-434.4132922
AGAGTAC7350.032.30635511
CAACGCA7500.032.1190725
CAGAGTA7400.032.0880710
GCAGAGT7550.031.450569
AACGCAG7700.030.8378856
TCAACGC8000.030.111634
TCCAGTA703.9009872E-529.4971072
Produced by FastQC (version 0.11.5)