FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262847
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35300.039.4501571
GGTATCA14750.036.832691
TATCAAC41050.034.005322
TGGTATC6550.033.069932
GTGGTAT6600.032.8221
TATACAC1900.032.572643
ATCAACG44700.031.1516823
CAACGCA45150.030.9173495
CTCGTAT1800.030.78385239
AACGCAG45500.030.6795256
TCAACGC45450.030.561984
TCGTATG1850.029.97217640
CAGAGTA45750.029.83078210
AGAGTAC45850.029.69074211
TTATACA2250.029.033882
CGCAGAG48300.028.8987148
GCAGAGT48150.028.629479
ACGCAGA48750.028.5614347
CTTATAC2300.028.4049591
AGACGTA2450.025.27542725
Produced by FastQC (version 0.11.5)