FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1795298
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38540.21467188177116No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37430.20848906421106692No Hit
GTATGATAACTGGCCACTTCTCAGTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT28850.16069755550332035No Hit
CTCCTGTACAGCCTCTGGATTCACCTTTAGTAGCTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAAGGG26410.14710649708293552No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26190.1458810737827369No Hit
GTAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA25500.1420377007048412No Hit
GTTATCATACTGCTGACAGTAATAAACTGCAACATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT24590.13696890432674688No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATG23380.1302290761756544No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22240.1238791554382615No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20800.11585820292787047No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19570.10900697265857813No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19310.10755874512197974No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18810.10477369216698286No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18280.10182153603468616No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATATCAATGGCCCTAGT18200.10137592756188667No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATGTTGCAGTTTATTACTGTCAGCAGTATGATAACTGG18100.10081891697088728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42800.039.153891
TATCAAC46550.035.8471762
GGTATCA17450.035.619021
ATCAACG50100.033.2348673
TCAACGC50100.033.0996634
CAACGCA51100.032.588115
AACGCAG51850.032.2491726
AGAGTAC53650.030.46320311
CGCAGAG55050.030.1259178
ACGCAGA56500.029.5958617
CAGAGTA57200.028.81347510
GCAGAGT58000.028.5344499
AGTACGG45900.025.20829413
GAGTACG46850.024.7704212
TGTTAGA6700.024.1430670
GTGGTAT10000.024.037921
TACCGTA750.00204943322.8907077
GAGTACT20800.022.77938712
TACGGGG23800.022.65025715
GCGTTGT10150.022.56627862
Produced by FastQC (version 0.11.5)