FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870558
Sequences flagged as poor quality0
Sequence length20-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC1450.042.7182121
GTATCAA33400.040.284581
GGTATAC350.001364683839.327881
TATACAC1850.039.0531733
TTATACA1700.038.4515152
TATCAAC36200.037.540262
GATCTAA400.00251591134.72110441
AACGCAG40050.034.0174266
GTATAAG2700.033.137381
CAGAGTA39650.033.05526410
CAACGCA41350.032.864755
AGAGTAC39700.032.8422211
GGTATCA13450.032.7488671
TAAGAGA2750.032.5274054
ATCAACG42650.032.105013
GCAGAGT42150.031.992449
ACGCAGA43300.031.4605397
CTATACT553.3100336E-431.276354
TCAACGC44050.031.240854
CGCAGAG43600.031.086278
Produced by FastQC (version 0.11.5)