FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310032
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACAGACTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA61060.4660954846904503No Hit
GTCTGTAGACCTTGCATGCAGTAATAAATTCCCACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGT43720.33373230577573676No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34930.26663470815980067No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30400.23205540017343088No Hit
GTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTT29160.2225899825347778No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27740.21175055265825565No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25820.19709442212098635No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22220.16961417736360637No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21480.16396546038570048No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21220.16198077604211195No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20980.16014875972495327No Hit
ATCCATTACTATGCAAGAGGCTCTGACTAGACCTACAGGAGATGTCGGCC20850.159156417553159No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAAGCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA19650.14999633596736567No Hit
GTGTAAAGTCTGTGCCGGATCCACTGCCAGTGAACCTGTCAGGGACCCCG19100.14579796524054375No Hit
GTCCCTGACAGGTTCACTGGCAGTGGATCCGGCACAGACTTTACACTGAA18690.142668270698731No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT18420.14060725234192753No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18240.13923324010405852No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC18160.13862256799833897No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17680.13495853536402164No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17190.13121816871648936No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16300.12442444154035932No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16240.12396643746106965No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16120.12305042930249033No Hit
CCCCTGGAGATCCGGCCGACATCTCCTGTAGGTCTAGTCAGAGCCTCTTG16080.12274509324963054No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16040.12243975719677076No Hit
GACTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTGGGAATTTATTACTGCATGCAAGGTCTACAGACT15830.12083674291925695No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG15260.11648570416600511No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14860.11343234363740734No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14550.11106598922774405No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGATCCGGCCGACATCTCCT13880.10595161034234277No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13740.10488293415733356No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTTCTTATCGGGCC13620.10396692599875423No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13450.10266924777410018No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13370.10205857566838061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8250.036.2776531
GTGGTAT3050.034.965181
GTATCAA16400.034.610991
TCGTATG1056.2391337E-1032.95182840
CTGTCTT24150.030.37034270
ACCGTAC1151.7462298E-929.932427
TATCAAC19450.029.1768742
TATACAC1452.3646862E-1128.4634593
ATCAACG20800.027.779243
TGGTATC4000.027.5146752
TCAACGC21250.026.8672734
CAACGCA21400.026.8396685
GTATGTA5950.026.5959071
AACGCAG21700.026.468616
TATGTAC5500.025.6386762
AGAGTAC22750.024.78587311
GTCTAAG1151.7408383E-623.9312881
GTACTAG1452.0467269E-823.75855430
CAGAGTA24000.023.3516810
CGCAGAG24750.023.2006238
Produced by FastQC (version 0.11.5)