FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526426
Sequences flagged as poor quality0
Sequence length20-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21450.039.4038281
TAAATCG350.001371196739.29168333
CTTATAC1350.038.0212361
GTATCAA50100.037.0877841
TATCAAC57550.032.6370122
TATACAC1700.032.1998943
AACGCAG65550.028.810436
ATCAACG67300.028.112113
ACTAATT1156.2257814E-428.07750570
CAACGCA67950.027.8431955
GTGGTAT8250.027.7900711
CAGAGTA65650.027.77093510
AGAGTAC65800.027.70762811
GCAGAGT67850.027.626689
TCAACGC70050.026.9596544
CGCAGAG70300.026.7125328
TCTATAC907.3722113E-626.6096363
TATACCT659.092109E-426.317225
ACGCAGA72150.026.266367
TACACAT2350.026.2052335
Produced by FastQC (version 0.11.5)