FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526426
Sequences flagged as poor quality0
Sequence length20-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28650.1876933437978651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22000.039.641851
TATACTG451.0568765E-437.9859625
GTATCAA50950.037.5906831
TATCAAC60000.031.9103012
AACGCAG66400.028.9356946
GTGTATA1950.028.0622021
CAACGCA69000.027.8949075
CAGAGTA67100.027.82045410
CGATTAA500.007487573727.7849741
ATCAACG69500.027.692413
AGAGTAC67150.027.59607711
GCAGAGT68650.027.5409349
TTATACC500.00808961627.3480993
TCAACGC72800.026.484084
ACGCAGA72300.026.480717
CGCAGAG72050.026.3361998
GTGGTAT9100.025.1808151
TACTATG700.001406171124.4211462
TGGTATC9300.023.895962
AGTACGG61900.022.09199513
Produced by FastQC (version 0.11.5)