FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006055
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATTACTGTCAGCAGTATGCTAGCTCACCTCGCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAA39500.39262266973475607No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35800.3558453563671966No Hit
GTAATAGACTGCACAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCG28000.27831480386261187No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25090.24938994388974756No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24250.2410404997738692No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23950.2380585554467698No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22970.22831753731157842No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22230.2209620746380665No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21440.21310962124337138No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT21300.21171804722405832No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21000.2087361028969589No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20420.20297101053123337No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20090.19969087177142403No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19770.19651013115585134No Hit
ATGCTAGCTCACCTCGCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAA18700.18587452972253007No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18170.1806064280779878No Hit
GTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTCGGTGCATCCACCAGGGCCA17830.1772268911739418No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17540.174344344991079No Hit
CTATTACTGTCAGCAGTATGCTAGCTCACCTCGCACTTTCGGCGGGGGGA17350.17245578025058272No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT16860.167585271182987No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16350.16251596582691802No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16300.1620189751057348No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16010.159136428922872No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCCGCCGAAAGTG15540.1544647161437496No Hit
GTAATACACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGAAACA15180.1508863829512303No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGT14920.14830203120107746No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14500.1441273091431383No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTGTGCAGTCTATTACTGTCAGCAGTATGCTAGCTCA14320.14233814254687865No Hit
CAGTAATAGACTGCACAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCG14270.1418411518256954No Hit
GTATGCTAGCTCACCTCGCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT14250.14164235553722213No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14080.13995258708519911No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13850.13766642976775623No Hit
CTCCTGTTGGGCCAGTCAGACTATTACCGATAGGTATTTAGCCTGGTACC13850.13766642976775623No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13700.13617545760420655No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13650.1356784668830233No Hit
GACTATTACCGATAGGTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGG13610.13528087430607671No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13510.13428689286371023No Hit
GTGTATTACTGTGCGAGAGATCAATCAGTAGTACCAGCTACTATAAGGTGGGGACCGAAAGACTACTACTATTT13340.13259712441168722No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13250.1317025411135574No Hit
GTCCCAGACCCACTGCCGCTGAACCTGTCTGGGATGCCAGTGGCCCTGGT12440.123651291430389No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12440.123651291430389No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA12360.12285610627649582No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12320.12245851369954923No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12160.12086814339176287No Hit
CACCAGGGCCACTGGCATCCCAGACAGGTTCAGCGGCAGTGGGTCTGGGA11600.11530184731451064No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTTGGGCCAGTCAGA11580.11510305102603734No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11350.11281689370859446No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11210.1114253196892814No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11160.11092832896809816No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT11090.11023254195844163No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11010.10943735680454846No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC10820.10754879206405217No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC10800.10734999577557887No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10720.10655481062168569No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10630.10566022732355587No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10540.10476564402542604No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG10440.10377166258305959No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGGGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG10410.10347346815034962No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10290.10228069041950986No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10260.10198249598679993No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10220.10158490340985334No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10200.10138610712138005No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC10200.10138610712138005No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10090.10029272753477693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA200.007472220351.55012513
TGTTAGA5200.043.1770270
GTATCAA12900.041.5680541
GGTATCA5050.039.4785731
TTAGGCT350.001341041539.46852537
TTAGTAT350.00137328539.2782363
TCGTATG1200.037.48026740
CTAGCGC1400.036.8599928
ACTAGCG1400.036.84715727
TATCAAC15250.035.157242
GACTAGC1500.034.3838426
CTTATAC1650.033.331961
CTCGTAT1350.033.29247339
GAGACTA1550.033.26972224
TATACAC1550.033.25983
AACGCAG16150.032.9852076
CAACGCA16350.032.581725
ATCAACG16300.032.470813
TCAACGC16350.032.3715134
TTATACA1600.032.220432
Produced by FastQC (version 0.11.5)