FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006055
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37750.3752279944933428No Hit
GTCTATTACTGTCAGCAGTATGCTAGCTCACCTCGCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAA37330.37105327243540365No Hit
GTAATAGACTGCACAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCG30610.30425771950837677No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25360.2520736937841371No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23340.23199526864833434No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23230.2309018890617312No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23080.2294109168981815No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22690.22553438927295227No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT22640.22503739855176905No Hit
GTCCCAGACCCACTGCCGCTGAACCTGTCTGGGATGCCAGTGGCCCTGGT21530.2140042045415012No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20630.20505837156020298No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19930.1981005014636377No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19500.19382638126146184No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19270.19154022394401898No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19060.18945286291504937No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17220.17116360437550632No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCCGCCGAAAGTG16980.16877804891382678No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16710.16609429901943729No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16010.159136428922872No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15860.1576454567593223No Hit
ATGCTAGCTCACCTCGCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAA15750.15655207717271918No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15320.15227795697054336No Hit
GTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTCGGTGCATCCACCAGGGCCA15310.1521785588263067No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15240.15148277181665018No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT15010.1491966144992073No Hit
CTATTACTGTCAGCAGTATGCTAGCTCACCTCGCACTTTCGGCGGGGGGA14570.14482309615279482No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGT14480.143928512854665No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14110.14025078151790907No Hit
GTAATACACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGAAACA14090.14005198522943577No Hit
CAGTAATAGACTGCACAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCG13910.1382628186331761No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13870.13786522605622953No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTGTGCAGTCTATTACTGTCAGCAGTATGCTAGCTCA13410.13329291142134375No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13390.13309411513287048No Hit
CTCCTGTTGGGCCAGTCAGACTATTACCGATAGGTATTTAGCCTGGTACC13310.1322989299789773No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13180.1310067541039009No Hit
GACTATTACCGATAGGTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGG13010.12931698565187788No Hit
GTATGCTAGCTCACCTCGCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT13000.12921758750764123No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA12980.12901879121916793No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12750.12673263390172504No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12610.125341059882412No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12370.12295550442073247No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11940.11868138421855665No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCGCTGAACCTGTCTGGGATGCCA11830.11758800463195353No Hit
GTGTATTACTGTGCGAGAGATCAATCAGTAGTACCAGCTACTATAAGGTGGGGACCGAAAGACTACTACTATTT11810.11738920834348023No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11640.11569943989145723No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11130.11063013453538822No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11060.1099343475257317No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11040.1097355512372584No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10860.10794638464099876No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10770.10705180134286893No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10640.10575962546779251No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC10630.10566022732355587No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10590.10526263474660928No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10590.10526263474660928No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10530.10466624588118939No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10480.10416925516000618No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTTGGGCCAGTCAGA10430.10367226443882294No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGGGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG10390.10327467186187635No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG10340.1027776811406931No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT10250.10188309784256327No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC10210.10148550526561669No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT10200.10138610712138005No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10180.10118731083290675No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10170.1010879126886701No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10160.10098851454443346No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAA350.00531597856.1802370
GGTATCA6050.040.3025251
GTATCAA14150.037.376061
TATAAGA1201.8189894E-1234.3337552
GTATAAG1500.032.0528031
TATCAAC16500.031.6286722
ATAAGAG1359.094947E-1230.5188923
ATCAACG17300.030.3645343
TCAACGC17600.029.8469584
AACGCAG18000.029.1851446
CGATTCA6800.028.91629470
CAACGCA18200.028.8629915
TACGGGT1950.028.17268815
AGAGTAC18700.027.90903911
CGCAGAG19000.027.6490828
TATGTAA500.00791892827.4670034
GCAGAGT20700.025.8760019
TGTTAGA6450.025.40449370
CAGAGTA21750.024.15322310
GAGTATG1001.6198685E-524.0396021
Produced by FastQC (version 0.11.5)