FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966803
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13630.14098011694212784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30100.039.500221
GGTATCA12600.037.635571
CGTTTAT400.002596011934.5003133
TATCAAC35350.033.6321142
GTGGTAT4700.032.9006581
AACGCAG38700.030.9031776
CAACGCA38850.030.7790835
TGGTATC4600.030.6262262
ATCAACG39550.029.9736793
TCAACGC40250.029.7085084
AGAGTAC39600.029.5065611
CAGAGTA39750.029.48167210
ACGCAGA41050.029.132557
GCAGAGT41700.028.6784429
CGAATTA605.551035E-428.6342815
CGCAGAG41650.028.5478558
ACCTATA658.133608E-426.8228548
TATAAGA1902.7284841E-1123.5102462
AGTACGG44100.023.3749213
GAGTACG45350.022.96149812
Produced by FastQC (version 0.11.5)