FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1528347
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33200.038.407291
GGTATCA14750.037.850621
CAACGCA40700.031.1573725
ATCAACG40950.031.1354563
TATCAAC40750.031.0345762
TCAACGC41050.030.9756644
AACGCAG41850.030.5482146
AGAGTAC43700.029.01272411
ACGCAGA44400.028.6347877
GTGGTAT5900.028.6216131
CAGAGTA44750.028.17800110
CGCAGAG45100.028.0366348
TGGTATC6750.026.546482
GCAGAGT48850.026.1656489
TCGTATG1251.2123746E-724.94954140
AGTACGG44150.023.57130613
GAGTACG45000.023.50587512
TATTAGA5050.021.153282
TTTAACG4850.020.65391234
GCGTTAT1851.1878001E-820.49740626
Produced by FastQC (version 0.11.5)