FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1528347
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22580.14774131790751707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32550.036.807721
GGTATCA15500.033.5394481
GTGGTAT7550.033.2879141
TGGTATC8350.029.6824552
TATCAAC40800.029.6142542
CAACGCA41050.029.1813725
ATCAACG41450.028.8978753
TCAACGC41950.028.5534424
AACGCAG42500.028.2686146
AGAGTAC43700.026.55140911
ACGCAGA45650.026.2468727
CAGAGTA44150.026.12481310
CGCAGAG46850.025.4276128
GCAGAGT47600.024.8099699
GTACTAA700.001350406324.5913641
TTAACGG3750.023.96254535
AATAGCG5600.023.84718362
TAATCGT5750.023.47709838
TAACGGC3900.023.05075336
TTTAACG3950.022.73879434
Produced by FastQC (version 0.11.5)