FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804907
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12550.1559186340782227No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTTG250.001450383277.9628670
TAAGCTA150.002363226868.94147526
TATTACA150.00237432268.860092
GGTATCA9600.041.618491
GTATCAA22750.039.514691
GTGGTAT3500.036.411051
TATCAAC25300.035.518742
TGGTATC3650.034.901692
CCCCTAG450.004659488330.6158961
CTTAAGC450.00467083330.6006813
CAGAGTA28350.030.35781710
AGAGTAC28650.030.28025811
AACGCAG29750.030.20216
GCAGAGT29950.029.5406429
CAACGCA30250.029.361485
ATCAACG32200.027.9041143
TAGTACT756.252298E-527.5406154
GGAACTA500.00781527827.5406136
GTGTATA500.00781527827.5406134
ACACTGA500.00781527827.5406136
Produced by FastQC (version 0.11.5)