FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893309
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26920.30135149203691No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22940.2567980396480949No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACTCAGGCTGCTATCCCATGTT22940.2567980396480949No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACTCAGG22460.25142475895798655No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20310.22735693920020958No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19700.2205283949898635No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17380.1945575383210065No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15760.1764227159918908No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15410.17250469882202016No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14850.16623587135022708No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG14270.15974315718301282No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14130.15817595031506457No Hit
CCCAAGCACTCAGGCTGCTATCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGA13640.15269072627724561No Hit
GTAATAGACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGGTCCAAGTAGG13550.15168323614785031No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC12200.13657088420692057No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12180.13634699751149937No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11640.13030205673512749No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11530.129070679910311No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAACCGACCCTCAGAGATTCCTGACCGATTCTCTGG11350.12705569965152036No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACTCAGGC10960.12268990909080732No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10730.12011521209346374No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10650.11921966531177901No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10550.11810023183467311No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10490.11742857174840958No Hit
GTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC10380.11619719492359307No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10310.11541359148961894No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10190.11407027131709185No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10180.11395832796938125No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGACCAGGGTGG10070.11272695114456475No Hit
GTCTGGAGTCCGGTGATGACCAGGGTGGCTGACGTGCCAGACTTGGAGCC10060.11261500779685416No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9830.1100403107995106No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9760.10925670736553646No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9710.10869699062698349No Hit
GGTCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAA9700.1085850472792729No Hit
GTTCCTGGGAGCTGCTGGTACCAGGATACATAATTATTCTCAATGTTGGA9510.10645812367277169No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9420.10545063354337636No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9240.10343565328458575No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC9220.10321176658916456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8350.046.713171
TGGTATC1700.042.635282
GTGGTAT1750.041.421771
GTATCAA19000.037.2432561
TCGTATG753.895184E-836.93140
CGCGGTT5400.034.01306570
CGTATGC901.9284198E-730.78447241
TATCAAC23150.030.7124862
ATAGGAC450.00461153930.6810723
ATCAACG23550.030.1925183
TAACGGC1052.2802851E-829.63675336
CAACGCA24150.029.585325
AACGCAG24200.029.5241936
TCAACGC24150.029.4423964
TTAACGG1103.5915946E-828.28328335
AGAGTAC24800.027.9701311
CAGAGTA25150.027.8553210
CGCAGAG26200.027.2673858
AATAGAC3950.027.088673
ACGCAGA27100.026.3618267
Produced by FastQC (version 0.11.5)