FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893309
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25770.288478007050192No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20960.23463325680139796No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18750.20989377695735742No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17970.20116219583593137No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17620.1972441786660607No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACTCAGGCTGCTATCCCATGTT15730.17608688594875904No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15640.17507939581936374No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG15430.17272858551744133No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14980.16769113487046475No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14820.1659000413070953No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC14810.1657880979593847No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACTCAGG14590.1633253443097517No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14210.15907149709674928No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT13420.15022797262761262No Hit
GTAATAGACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGGTCCAAGTAGG12460.13948141124739594No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12210.13668282755463115No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11580.12963039664886394No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAACCGACCCTCAGAGATTCCTGACCGATTCTCTGG11320.12671986960838857No Hit
GTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC10970.12280185243851792No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10720.12000326874575314No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10520.11776440179154134No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10250.1147419314033554No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10120.11328666788311773No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10090.11295083783998594No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC10060.11261500779685416No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9970.11160751766745886No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9840.11015225414722117No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9830.1100403107995106No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9700.1085850472792729No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9630.10780144384529876No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9520.10657006702048227No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9420.10545063354337636No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9400.10522674684795517No Hit
GGTCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAA9390.1051148035002446No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGAAGCTCCAACATTGAGAATAATTATGTATCCTGGTACCAGCAG9250.10354759663229633No Hit
GGATAGCAGCCTGAGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC8940.10007735285326802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATATT200.00738032451.7112515
CTTAGCG200.007381957551.708363
GTGGTAT2750.045.1373981
GGTATCA8750.042.9521751
TGGTATC3250.039.249712
GTATCAA20150.037.9878461
CGCGGTT4950.036.54433470
TATCAAC23800.032.3032342
ATCAACG25350.030.4606763
AACGCAG25250.030.4481986
TCAACGC25400.030.4007134
CAACGCA25500.030.2848875
TATAAGA2200.029.774812
TGTGTCG2401.6734703E-1029.61069970
AGAGTAC25900.029.15160211
CAGAGTA25950.028.9625810
CGCAGAG26600.028.9028938
AATAGAC4050.028.088493
CCTAAGA756.186433E-527.5839651
TACACTC756.191153E-527.5808755
Produced by FastQC (version 0.11.5)