FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683256
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC200.00754141251.4283033
TCTTATA200.00754141251.4283032
GACCTAG200.00754796251.417017
ACTCATA250.00974738648.1943669
GATACTT650.001168614740.58168470
TTAATGG350.001389374739.183474
TATGAGA350.001389374739.183472
TACTCAG350.001389374739.183475
GTATCAA19400.039.0600931
GGTATCA8000.039.002651
GTATAAA451.03813356E-438.097831
TATCAAC21600.035.396642
TATACAC601.4002875E-534.2855383
TATAAAC501.9300677E-434.2855382
AGAGTAC24300.030.89252711
CAGAGTA24200.030.7368910
CTACACT450.00476452230.4760324
TGTGTAT450.00476964230.46934312
GTGGTAT2600.030.3317341
AACGCAG25500.030.2519426
Produced by FastQC (version 0.11.5)