FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683256
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17840.26110271991757117No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG252.6529827E-454.8306434
TGTTTAA200.007171828852.08601443
CCTTATA200.007551303651.4112552
CTTATAG200.007555674851.4037323
CTAGGAC200.007555674851.4037323
GTATCAA20400.040.6645281
TATCAAC22700.036.5389862
GGTATCA7750.036.2695961
GTGGTAT3250.035.8613051
TGGTATC3600.034.274172
ATTACAC400.002682469334.2691543
AACGCAG26200.031.653196
CAGAGTA25450.031.6457410
AGAGTAC25550.031.38774911
GCAGAGT26650.030.9923889
CAACGCA27400.030.6421265
TACAAGT450.004775671830.4614734
ATCAACG27600.030.2959183
TACAGGG704.0251194E-529.377862
TCAACGC28750.029.3224754
Produced by FastQC (version 0.11.5)