FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890714
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC751.8189894E-1145.760171
GGTATCA12000.044.3301661
TTATACA857.094059E-1140.3743552
TATACAC857.094059E-1140.3743553
GTATCAA28850.039.494911
TATCAAC31600.035.947232
GCTTAGG400.002663422134.320131
ATATGCT400.002664155634.3182034
AACGCAG36450.031.4465856
GTGGTAT3550.030.9364551
ATACAGT450.00474314630.505074
AGAGTAC36100.030.41544511
CAGAGTA36300.030.34239210
GCAGAGT37900.029.9667859
ATACACA1151.8007995E-929.8419154
TGGTATC3800.029.802652
CAACGCA38800.029.541965
ATCAACG38700.029.4409393
TCAACGC39850.028.7635614
TATGCTG605.5914384E-428.5985035
Produced by FastQC (version 0.11.5)