FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108602
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17970.1620960452894727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13000.038.8098451
GTATCAA29550.038.4448741
GTGGTAT5650.038.2701381
TGGTATC5750.036.992652
TATCAAC32950.034.5682
TGTATAA501.9225052E-434.3107917
CTCTATA501.9235245E-434.3076972
TGATACT450.00474865230.4984845
TAAGAGA902.0952575E-730.4957334
TCTATAT450.00475074830.4957333
CAGAGTA37100.030.3354410
AGAGTAC37250.030.21328211
AGTAGTA802.8844934E-630.0192382
AACGCAG38650.029.8291026
GCAGAGT38950.029.599359
TATAAGA953.364239E-728.8906942
CAACGCA40800.028.4241375
ACGCAGA41950.027.5656267
CTATATC500.00794865827.446164
CTAACCC500.00794865827.446164
Produced by FastQC (version 0.11.5)