FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1343946
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22400.16667336336430183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32000.037.373221
TATCAAC38550.030.9250622
GGTATCA16600.030.7583621
AACGCAG39200.030.3248836
ATCAACG39450.030.0447543
TCAACGC39700.029.769274
CAACGCA39850.029.7442845
GTGGTAT6500.029.5229471
AGAGTAC40550.028.47259911
ACGCAGA41950.028.4207387
CAGAGTA41600.027.5892310
CGCAGAG43350.027.344828
GCAGAGT46350.025.6488469
AGTACGG38050.023.77044513
GAGTACG40050.023.01112412
AGTATCG800.003014940821.41811421
CGATTAC1154.204353E-521.24847647
TACGGGA13300.021.12195415
GTACGGG41150.020.9790814
TTATACG850.004116872320.29722635
Produced by FastQC (version 0.11.5)