FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845768
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGAAT252.6001828E-455.055774
CTAAACG400.00922871148.86415570
TTATACA851.8189894E-1244.5304032
GGTATCA12000.042.4513741
GTGGTAT3900.040.597991
GTATCAA28800.040.3957371
TACACAT1050.039.325555
TTCTTAT3550.036.70546770
TATCAAC31800.036.6822052
CTTATAC1051.6370905E-1136.0590821
TATACAC1051.6370905E-1136.0484243
ATCAGTA1253.6379788E-1233.09608531
TTAAACT2400.032.9761124
TAAACTA2450.032.303135
ATACACA1305.456968E-1231.7629434
ATTAAAC2500.031.6570663
AACGCAG37050.031.5771986
CAATTAA2450.030.9077841
CAGAGTA36800.030.57072610
AGAGTAC36900.030.4878811
Produced by FastQC (version 0.11.5)