FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155248
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC454.5110937E-1061.1183783
TCTAACG200.007461904551.5686262
CTTATAC552.6793714E-950.008111
TTATACA605.7880243E-945.8387762
GGTATCA14150.042.03421
GTATCAA35000.039.8814661
CTAAGAC702.2077074E-839.3909830
CGTTTAG350.00135415439.3909830
ACAGTAA350.001355888239.38072629
ATACACA754.146932E-836.6710244
TATCAAC38700.036.2446142
ATGTGTA952.2737368E-1036.18694711
CTCAATT750.002406780135.03476370
TGGTATC4950.034.031822
GTGGTAT5300.032.43451
CCTAAGA751.6851372E-632.16092729
ACCCTAA751.69544E-632.13583427
GTGTATA1101.1004886E-931.25236113
TGTGTAT1101.1004886E-931.25236112
TAAGACA902.0020161E-730.65472231
Produced by FastQC (version 0.11.5)