FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155248
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17300.14975139537138346No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTA150.00238529268.78223
CAATATT350.005202902556.48726370
CCTAGAC405.1185E-542.957123
GGTATCA14150.041.776761
GTATCAA36800.041.6533361
TATCAAC40050.038.1857572
GTGGTAT4900.037.1742741
TGGTATC5000.035.0545272
CAGAGTA45400.033.08316810
AGAGTAC45200.033.07747311
GCAGAGT46700.032.6774069
AACGCAG48150.031.9047346
ATAAGAG1305.456968E-1231.7221783
CAACGCA49050.031.1778495
CAATGTA450.00450975830.822140
GTATAAG1359.094947E-1230.5499291
GTTCTAG450.00470975130.5499271
CCTTACT450.004710748330.5486052
CGCAGAG50600.030.3625688
ATCAACG50500.030.1465423
Produced by FastQC (version 0.11.5)