FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962929
Sequences flagged as poor quality0
Sequence length20-76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21670.2250425524623311No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACT306.4495805E-445.7975924
GTGGTAT6650.040.8109781
GGTATCA18900.040.7153131
GATATAG350.001266355939.93103450
GTATCAA45300.039.3586961
GTGTATA1050.039.2611921
TGGTATC6950.039.04532
TATCAAC49850.035.624752
TATCATT400.00265287634.3481944
AGGAGTA1850.033.42162
AACGCAG56000.031.6494066
CAACGCA55900.031.6445795
CAGAGTA55600.030.9520510
AGAGTAC55850.030.87500411
TCAACGC57400.030.8176294
GCAGAGT57100.030.6803639
CTACATT450.004723113530.5317274
ATCAACG58450.030.381553
ACGCAGA59650.029.7143147
CGCAGAG58900.029.6844448
Produced by FastQC (version 0.11.5)