FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1383687
Sequences flagged as poor quality0
Sequence length20-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42700.042.475451
CTTATAC2100.040.9709431
AGGGGGG1900.040.76304270
TATACAC2350.039.5427473
TATCAAC48050.037.746132
GGTATCA16050.037.0959361
TTATACA2500.034.4155922
AACGCAG53700.033.5836526
CAGAGTA53350.032.70137410
CAACGCA55800.032.5047875
ATCAACG56100.032.3923153
AGAGTAC54200.032.25201811
GCAGAGT56450.031.5772259
TCAACGC58250.031.2558044
ACGCAGA59900.030.0468337
TGGTATC6200.029.974872
CGCAGAG59800.029.9244378
GTGGTAT6050.029.5803431
CGCTAGT500.006865626728.28275766
GTCTAGG500.00782846927.5324751
Produced by FastQC (version 0.11.5)