FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073219
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATTAA200.00430057859.27416269
CTAAGCT252.5812158E-455.138793
CTTATAC653.6379788E-1253.0254821
TTATACA653.6379788E-1253.018072
TATACAC653.6379788E-1253.018073
GTTTGTA200.00696235252.48002658
ATAGGTT200.007381723351.70948424
TATAGAC405.0346538E-543.0771832
GGTATCA11100.042.8503231
GTATCAA27700.041.1856771
GTGGTAT3150.040.4845351
ATACACA955.456968E-1239.9030724
TAAGCTC350.001355148639.384854
TATCAAC31000.036.9074172
TGGTATC3400.036.4889072
GAGACTA952.2191671E-1036.28735724
AGGGGGG1101.0338696E-535.83629670
ATATAGA400.002608632434.4665641
TAGGAGT400.002610421534.4617464
TGTGTAT1056.511982E-1032.8161212
Produced by FastQC (version 0.11.5)