FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928785
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATAAT309.178371E-657.443052
GTAACAG350.005821052554.90579270
CTTATAC200.007387478451.6987421
ACTTAAT200.00739849751.67925615
GTGGTAT3650.042.4921151
GTCATAA503.8682574E-641.3589941
GGTATCA10700.040.5859261
GTATTGT350.001354265539.3895151
TATGAAT350.001354265539.3895152
CATAATC451.0072777E-438.2953643
GTATCAA24500.037.7013931
ATAATCA501.872013E-434.4658284
TGTAGGA601.3514107E-534.4658282
TATCAAC27450.033.398582
TGGTATC5600.028.3112142
AACGCAG33200.028.0294366
AGAGTAC31850.027.90691811
CAGAGTA32050.027.84026310
CAACTCA500.007771654527.5726645
TATACAC500.007771654527.5726643
Produced by FastQC (version 0.11.5)