FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928785
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11290.1215566573534241No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCTA200.00731141451.8342434
GTGGTAT4700.051.2975651
TCATAAT552.6411726E-950.087622
ACACTAT400.00940304148.6340170
ATAACGT306.36953E-445.9136473
GTTAGAA350.001311575839.64697343
GTATCAA26200.039.5696141
TGCTATT350.001350855739.40971829
ATAAGAG952.237357E-1036.2476163
GGTATCA11000.036.008231
TATCAAC28950.035.5652352
GTCATAA807.196286E-834.4426541
GTATAAG905.2732503E-934.4426541
TAATCAA807.209928E-834.4352345
CTAACAC400.002620109834.4352343
TGGTATC7350.034.200982
CTAGTAT450.00408845931.44538766
GCTAGTA450.004125113631.388165
TAAGAGA1001.4761099E-830.9917134
TATGTCA450.00453011530.79311439
Produced by FastQC (version 0.11.5)