FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975304
Sequences flagged as poor quality0
Sequence length20-76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT252.5982776E-455.064791
AGACTTA252.5995943E-455.05914320
TTTTACC200.007010198752.38884750
TTATACA1250.052.303512
TATACAC1400.046.7019543
CTTATAC1450.045.096171
TTAAAGG1001.3594945E-744.33934470
TACACAT1500.043.588495
GGTATCA13200.043.2800941
GTATCAA33050.041.027851
TCGCTTA654.4602893E-738.1163166
TATCAAC36950.036.7849272
GTCATAA754.10746E-836.709861
ATACACA1800.036.3237424
TGACATA400.00252286834.7019841
CCTAAAG400.00262887334.4119643
GTGGTAT4100.032.736691
GTGTATA959.0058165E-932.60415613
CTTAAAG851.5713213E-632.44857869
TGTATAA1001.48447725E-830.97394414
Produced by FastQC (version 0.11.5)